SIMILAR PATTERN OF AMINO ACIDS MAPPED TO '4xwl'

List of Similar Pattern of Amino Acids

Hit pattern: 3D amino acid arrangements similar to known drug binding site
Query pattern: Residues from known binding site for annotated drug that match the hit pattern

(Click on the DrReposER ID to view details on interfaces and similar patterns of amino acids)
(Click on the view link on the last column to view superposed patterns of amino acids)
Filter list by:
DrReposER ID / Desc. Hit
PDBID		 Hit
Macromolecule		 Res.
Matches		 Interface HETATM RMSD Dali
Z-score		 Seq.
Identity (%)		 View Dock
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1A4L_C_DCFC1353_2
(ADENOSINE DEAMINASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 5 LEU A 324
SER A 262
LEU A 288
GLY A 256
 None
 0.91A 1a4lC-4xwlA:
undetectable		 1a4lC-4xwlA:
18.62
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1AV2_C_DVAC6_0
(GRAMICIDIN A)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 ALA A 598
VAL A 593
TRP A 590
 None
 0.70A 1av2C-4xwlA:
undetectable
1av2D-4xwlA:
undetectable		 1av2C-4xwlA:
2.84
1av2D-4xwlA:
2.84
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1CMC_A_SAMA105_0
(MET REPRESSOR)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		5 / 10 ALA A 108
GLY A 372
LEU A 120
HIS A 110
PRO A 117
 EDO  A 717 ( 3.8A)
None
None
EDO  A 717 (-4.1A)
None
 1.38A 1cmcA-4xwlA:
undetectable
1cmcB-4xwlA:
undetectable		 1cmcA-4xwlA:
9.70
1cmcB-4xwlA:
9.70
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1K6C_B_MK1B902_1
(POL POLYPROTEIN)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		5 / 10 VAL A 185
ILE A 571
GLY A 563
GLY A 197
PRO A 188
 None
 0.84A 1k6cA-4xwlA:
undetectable		 1k6cA-4xwlA:
9.86
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1KXH_A_ACRA598_2
(ALPHA-AMYLASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 TRP A 354
TYR A 385
LEU A 287
 None
P33  A 705 (-3.9A)
None
 0.83A 1kxhA-4xwlA:
undetectable		 1kxhA-4xwlA:
20.83
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1N2X_B_SAMB402_0
(S-ADENOSYL-METHYLTRA
NSFERASE MRAW)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		5 / 12 GLY A 314
GLY A 364
ASP A 222
GLY A 405
ASN A 494
 None
 0.90A 1n2xB-4xwlA:
undetectable		 1n2xB-4xwlA:
17.47
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1TT0_A_ACTA4901_0
(PYRANOSE OXIDASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 6 GLN A 407
PHE A 313
HIS A 312
ASN A 401
 None
 1.07A 1tt0A-4xwlA:
undetectable		 1tt0A-4xwlA:
22.76
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1TT0_B_ACTB3901_0
(PYRANOSE OXIDASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 6 GLN A 407
PHE A 313
HIS A 312
ASN A 401
 None
 1.06A 1tt0B-4xwlA:
0.9		 1tt0B-4xwlA:
22.76
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1TT0_C_ACTC6901_0
(PYRANOSE OXIDASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 6 GLN A 407
PHE A 313
HIS A 312
ASN A 401
 None
 1.07A 1tt0C-4xwlA:
undetectable		 1tt0C-4xwlA:
22.76
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1TT0_D_ACTD5901_0
(PYRANOSE OXIDASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 6 GLN A 407
PHE A 313
HIS A 312
ASN A 401
 None
 1.07A 1tt0D-4xwlA:
undetectable		 1tt0D-4xwlA:
22.76
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1VE9_A_BEZA352_0
(D-AMINO ACID OXIDASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 5 TYR A 374
TYR A 267
ILE A 306
GLY A 372
 None
 1.32A 1ve9A-4xwlA:
undetectable		 1ve9A-4xwlA:
19.25
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
1W5U_C_DVAC6_0
(GRAMICIDIN D)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 ALA A 598
VAL A 593
TRP A 590
 None
 0.69A 1w5uC-4xwlA:
undetectable
1w5uD-4xwlA:
undetectable		 1w5uC-4xwlA:
2.84
1w5uD-4xwlA:
2.84
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2AOX_A_THAA400_1
(HISTAMINE
N-METHYLTRANSFERASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 8 PHE A 577
PHE A 551
TYR A  43
TYR A 157
 None
 1.35A 2aoxA-4xwlA:
undetectable		 2aoxA-4xwlA:
16.71
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2BTE_A_LEUA1894_0
(AMINOACYL-TRNA
SYNTHETASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 8 TYR A  54
ASP A 615
SER A 491
TYR A 498
 None
 1.27A 2bteA-4xwlA:
3.7		 2bteA-4xwlA:
20.70
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2DU8_G_BEZG2352_0
(D-AMINO-ACID OXIDASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 5 TYR A 374
TYR A 267
ILE A 306
GLY A 372
 None
 1.34A 2du8G-4xwlA:
undetectable		 2du8G-4xwlA:
19.15
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2DU8_J_BEZJ3352_0
(D-AMINO-ACID OXIDASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 5 TYR A 374
TYR A 267
ILE A 306
GLY A 372
 None
 1.32A 2du8J-4xwlA:
undetectable		 2du8J-4xwlA:
19.15
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2FCN_A_DVAA35_0
(UBIQUITIN)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 4 GLN A 348
GLU A 350
ILE A 349
PRO A 318
 None
 1.42A 2fcnA-4xwlA:
undetectable		 2fcnA-4xwlA:
7.04
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2HA4_B_ACHB603_0
(ACETYLCHOLINESTERASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 7 TYR A 116
GLU A 109
HIS A 310
GLY A 372
 None
 1.25A 2ha4B-4xwlA:
undetectable		 2ha4B-4xwlA:
22.63
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2HDN_F_TACF3888_1
(ELONGATION FACTOR
EF-TU)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 7 SER A 180
ASP A 397
VAL A  38
LEU A 597
 None
 0.86A 2hdnE-4xwlA:
undetectable
2hdnF-4xwlA:
undetectable
2hdnH-4xwlA:
undetectable		 2hdnE-4xwlA:
6.14
2hdnF-4xwlA:
19.17
2hdnH-4xwlA:
19.17
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2IZQ_B_DVAB6_0
(GRAMICIDIN D)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 TRP A 590
ALA A 598
VAL A 593
 None
 0.84A 2izqA-4xwlA:
undetectable
2izqB-4xwlA:
undetectable		 2izqA-4xwlA:
2.84
2izqB-4xwlA:
2.84
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2QMM_B_SAMB301_0
(UPF0217 PROTEIN
AF_1056)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		5 / 11 LEU A 492
GLY A 487
GLY A 612
LEU A 496
SER A 497
 None
 0.87A 2qmmA-4xwlA:
undetectable
2qmmB-4xwlA:
undetectable		 2qmmA-4xwlA:
15.14
2qmmB-4xwlA:
15.14
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
2Y7K_A_SALA1303_1
(LYSR-TYPE REGULATORY
PROTEIN)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 7 PRO A  91
SER A  92
PRO A  85
GLY A  86
 None
None
None
PGE  A 707 ( 4.5A)
 0.83A 2y7kA-4xwlA:
undetectable		 2y7kA-4xwlA:
14.83
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3D91_A_REMA350_2
(RENIN)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 5 TYR A   4
ALA A 500
ALA A 619
MET A  61
 None
 1.28A 3d91A-4xwlA:
undetectable		 3d91A-4xwlA:
19.12
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3D91_A_REMA350_2
(RENIN)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 5 TYR A 352
ALA A 320
ALA A 415
TYR A 419
 None
None
None
EDO  A 721 (-4.3A)
 1.29A 3d91A-4xwlA:
undetectable		 3d91A-4xwlA:
19.12
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3DL9_A_V2HA602_0
(CYTOCHROME P450 2R1)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		5 / 12 THR A 622
ALA A 408
TYR A 419
VAL A 441
ILE A 525
 None
None
EDO  A 721 (-4.3A)
None
None
 1.18A 3dl9A-4xwlA:
undetectable		 3dl9A-4xwlA:
19.65
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3HUO_A_PNNA303_0
(CTX-M-9
EXTENDED-SPECTRUM
BETA-LACTAMASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 4 GLN A 348
GLN A 316
GLY A 314
HIS A 312
 None
 1.46A 3huoA-4xwlA:
undetectable		 3huoA-4xwlA:
15.60
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3KHM_A_TPFA501_1
(STEROL 14
ALPHA-DEMETHYLASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 8 TYR A  55
ALA A 234
ALA A  63
THR A  66
 None
 0.99A 3khmA-4xwlA:
undetectable		 3khmA-4xwlA:
20.26
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3L8L_A_DVAA6_0
(GRAMICIDIN D)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 ALA A 598
VAL A 593
TRP A 590
 None
 0.81A 3l8lA-4xwlA:
undetectable
3l8lB-4xwlA:
undetectable		 3l8lA-4xwlA:
2.84
3l8lB-4xwlA:
2.84
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3L8L_C_DVAC6_0
(GRAMICIDIN D)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 ALA A 598
VAL A 593
TRP A 590
 None
 0.73A 3l8lC-4xwlA:
undetectable
3l8lD-4xwlA:
undetectable		 3l8lC-4xwlA:
2.84
3l8lD-4xwlA:
2.84
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3LSK_B_ACTB901_0
(PYRANOSE 2-OXIDASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 6 GLN A 407
PHE A 313
HIS A 312
ASN A 401
 None
 1.08A 3lskB-4xwlA:
undetectable		 3lskB-4xwlA:
22.76
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3LSK_D_ACTD901_0
(PYRANOSE 2-OXIDASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 6 GLN A 407
PHE A 313
HIS A 312
ASN A 401
 None
 1.05A 3lskD-4xwlA:
undetectable		 3lskD-4xwlA:
22.76
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3PMZ_E_TUBE220_1
(SOLUBLE
ACETYLCHOLINE
RECEPTOR)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 6 TYR A 374
GLN A 375
SER A 367
SER A 309
 None
 1.14A 3pmzD-4xwlA:
undetectable		 3pmzD-4xwlA:
16.30
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3ROD_A_NCAA302_0
(NICOTINAMIDE
N-METHYLTRANSFERASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 8 LEU A 626
SER A 493
TYR A 623
SER A 522
 None
 1.05A 3rodA-4xwlA:
undetectable		 3rodA-4xwlA:
19.33
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
3TWP_D_SALD404_1
(ANTHRANILATE
PHOSPHORIBOSYLTRANSF
ERASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 5 ASN A 219
ALA A 220
PRO A 221
ALA A 225
 None
PGE  A 712 (-3.5A)
None
None
 1.28A 3twpD-4xwlA:
3.1		 3twpD-4xwlA:
19.16
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
4E7C_A_ACTA504_0
(UDP-N-ACETYLGLUCOSAM
INE
1-CARBOXYVINYLTRANSF
ERASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 ARG A 305
TRP A 304
GLY A 295
 None
PGE  A 709 (-4.1A)
None
 1.04A 4e7cA-4xwlA:
undetectable		 4e7cA-4xwlA:
21.56
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
4FFW_B_715B801_2
(DIPEPTIDYL PEPTIDASE
4)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 ARG A 260
TYR A 385
ASN A 311
 None
P33  A 705 (-3.9A)
None
 0.78A 4ffwB-4xwlA:
undetectable		 4ffwB-4xwlA:
20.77
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
4FFW_B_715B801_2
(DIPEPTIDYL PEPTIDASE
4)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 ARG A 269
TYR A 286
ASN A 311
 None
 0.83A 4ffwB-4xwlA:
undetectable		 4ffwB-4xwlA:
20.77
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
4PGF_A_ADNA502_2
(ADENOSYLHOMOCYSTEINA
SE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 6 THR A 172
THR A 184
HIS A  31
LEU A 150
 None
 1.13A 4pgfA-4xwlA:
undetectable		 4pgfA-4xwlA:
20.06
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
4RKU_A_PQNA5001_1
(PHOTOSYSTEM I P700
CHLOROPHYLL A
APOPROTEIN A1
PHOTOSYSTEM I
REACTION CENTER
SUBUNIT IX)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 8 SER A 262
ALA A 321
LEU A 324
PHE A  69
 None
 1.04A 4rkuA-4xwlA:
2.5
4rkuJ-4xwlA:
undetectable		 4rkuA-4xwlA:
20.08
4rkuJ-4xwlA:
5.17
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
4RTB_A_SAMA501_0
(HYDG PROTEIN)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		5 / 12 LEU A 324
PHE A 330
LYS A  73
ALA A  75
PHE A  72
 None
 1.39A 4rtbA-4xwlA:
undetectable		 4rtbA-4xwlA:
22.51
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
4ZE2_A_1YNA602_2
(LANOSTEROL 14-ALPHA
DEMETHYLASE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 5 ALA A 237
TYR A 233
HIS A   7
PRO A   0
 PG4  A 704 ( 4.0A)
None
None
PG4  A 704 (-4.3A)
 1.29A 4ze2A-4xwlA:
1.5		 4ze2A-4xwlA:
21.71
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5FUQ_A_ACTA1278_0
(NAD(P)H
DEHYDROGENASE
[QUINONE] 1)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		4 / 5 HIS A 310
SER A 309
ILE A 306
TYR A 267
 None
 1.37A 5fuqA-4xwlA:
undetectable
5fuqB-4xwlA:
undetectable		 5fuqA-4xwlA:
17.45
5fuqB-4xwlA:
17.45
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5GLM_A_ACTA613_0
(GLYCOSIDE HYDROLASE
FAMILY 43)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 ASP A  77
SER A 247
TYR A 248
 None
 0.88A 5glmA-4xwlA:
undetectable		 5glmA-4xwlA:
20.23
Potential target for repurposing, i.e. matched protein structure has less than 30% sequence identity to known drug target, and may potentially interact with the same drug molecule based on local structural similarity of binding site.
5XIP_A_HFGA1003_1
(PROLYL-TRNA
SYNTHETASE, PUTATIVE)		 4xwl EXOGLUCANASE S
(Clostridium
cellulovorans) 		3 / 3 GLU A  46
TRP A 149
HIS A  31
 None
PGE  A 712 ( 3.8A)
None
 0.74A 5xipA-4xwlA:
undetectable		 5xipA-4xwlA:
21.25